In-situ sulfite treatment promotes solid reduction during aerobic digestion of waste activated sludge: Feasibility for small-scale wastewater treatment plants.

Aerobic digestion remains the preferred choice for small-scale wastewater treatment plants (WWTPs) in some developing countries, largely due to economic viability and operational simplicity. The escalating production of waste activated sludge (WAS) has prompted small-scale WWTPs to improve efficiency. To address this issue, this study employed an in-situ sulfite treatment as a non-intrusive method to augment aerobic digestion. With sulfite-enhanced solubilization and hydrolysis, a 3.6-fold increase in degradation was achieved. Both sludge dewatering properties and pathogens inactivation were improved. Microbial community analysis revealed a preferential enrichment of Actinobacteriota and Firmicutes during sulfite treatment. The desktop scaling-up estimation suggests that implementing this treatment yielded operational cost savings exceeding 40 %. In summary, in-situ sulfite treatment offers a cost-effective strategy for WAS management in small-scale WWTPs.

Achieving efficient methane production from protein-rich organic waste in anaerobic digestion: Using conductive materials or regulating inoculum-to-substrate ratios?

The contribution of inoculum-to-substrate ratios (ISRs) and conductive materials (CMs) on the productivity of anaerobic digestion (AD) remains unclear, particularly for protein-rich organic waste. This study investigated whether the addition of CMs, i.e., biochar and iron powder, can overcome the limitations imposed by varying ISRs for the AD of protein as the sole substrate. Results indicate the ISR plays a decisive role in hydrolysis, acidification, and methanogenesis for protein conversion, irrespective of CMs addition. Methane production increased stepwise as the ISR escalated to 3:1. The addition of CMs provided limited improvement, and iron powder even inhibited methanogenesis at a low ISR. Bacterial community variations were contingent on the ISR, while iron powder supplementation significantly elevates the proportion of hydrogenotrophic methanogen. This study demonstrates that the addition of CMs could affect methanogenic efficiency but can not overcome the limitation of ISRs for the AD of protein.

Evaluating Fouling Control and Energy Consumption in a Pilot-Scale, Low-Energy POREFLON Non-Aerated Membrane Bioreactor (LEP-N-MBR) System at Different Frequencies and Amplitudes.

Continual aeration, a fouling control strategy that causes high energy consumption, is the major obstacle in the deployment of membrane bioreactors (MBRs) for wastewater treatment. In recent years, a technology has been developed which adopts mechanical reciprocity for membrane vibration, and it has been proven efficient for membrane scouring, as well as for saving energy: the low-energy POREFLON non-aerated membrane bioreactor (LEP-N-MBR). In this study, a pilot-scale LEP-N-MBR system was designed, established, and operated at various frequencies and amplitudes, and with various membrane models, so as to evaluate energy usage and membrane fouling. The results showed that a slower TMP rise occurred when the frequency and amplitude were set to 0.5 Hz and 10 cm, respectively. Under a suitable frequency and amplitude, the TMP increasing rate of model B (sealed only with epoxy resin) was slower than that of model A (sealed with a combination of polyurethane and epoxy resin). The average specific energy demand (SED) of the LEP-N-MBR was 0.18 kWh·m-3, much lower than the aerated MBR with 0.43 kWh·m-3 (obtained from a previous study), indicating a significant decrease of 59.54% in the SED. However, the uneven distribution of sludge within the membrane tank indicated that the poor hydraulic mixing in the reactor may result in sludge accumulation, which requires further operational optimization. The findings of this pilot-scale study suggest that the LEP-N-MBR system is promising and effective for municipal wastewater treatment with a much lower level of energy usage. More research is needed to further optimize the operation of the LEP-N-MBR for wide application.

Insights into the role of biochar on the acidogenic process and microbial pathways in a granular sulfate-reducing up-flow sludge bed reactor.

In this study, the effect of biochar on sulfate reduction and anaerobic acidogenic process was explored in a granular sulfate-reducing up-flow sludge bed reactor in both long-term operation and batch tests. Both bioreactors had a high sulfate reduction efficiency of over 95% during the long-term operation, while the reactor with biochar addition showed higher sulfate reduction efficiency and stronger robustness against volatile fatty acids accumulation with a higher organic loading and sulfate loading rate. Batch tests showed that adding biochar significantly lessened the lag phase of the sulfate-reducing process, accelerated the adaption of acidogens, and facilitated both production and utilization of volatile fatty acids. The microbial pathways proved that biochar could regulate the acidification fermentation pathway and facilitate the enrichment of assimilative desulfurization bacteria. Overall, this study revealed that the acidogenic sulfate-reducing metabolic pathway could be enhanced by biochar, offering a potential application for effective sulfate-laden wastewater treatment.